Biology
Cell Dilution Calculator
Find the stock volume and diluent volume needed to reach a target cell concentration using C₁V₁ = C₂V₂.
Your inputs
Change any value to update the result instantly.
Stock volume to use
1 mL
Diluent to add
9 mL
Dilution factor
10 ×
Final volume
10 mL
What this calculator does
Solves C₁V₁ = C₂V₂ to plan a dilution from a cell stock to a target concentration and volume.
What your result means
The headline volume of stock to combine with the stated diluent volume yields the target concentration.
Formula and working
V₁ = C₂ × V₂ ÷ C₁; diluent = V₂ − V₁
- 1
Collect the inputs
Stock concentration (C₁) = 2,000,000 cells/mL; Target concentration (C₂) = 200,000 cells/mL; Final volume wanted (V₂) = 10 mL
- 2
Apply the formula
V₁ = C₂ × V₂ ÷ C₁; diluent = V₂ − V₁
- 3
Result
Stock volume to use = 1 mL
Frequently asked questions
Symbols and assumptions
- C₁
- Stock concentration
- V₁
- Stock volume to use
- C₂
- Target concentration
- V₂
- Final volume
- • Concentration and volume units are consistent on both sides of the equation.
- • Volumes are additive.
Sources and review
Reviewed 2026-09-22 · Next review 2027-09-22
- • Cannot concentrate cells above the stock concentration; pipetting very small volumes increases error.
Important limitation
This is an educational estimate based only on the values and formula shown. Verify current rates, rules, units, and professional standards before relying on the result. It is not personalized financial or investment advice.
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