Chemistry
Protein Solubility Calculator
Express how much protein is dissolved in a solution as a concentration and as a percentage.
Your inputs
Change any value to update the result instantly.
Dissolved protein concentration
38 mg/mL
Protein dissolved
380 mg
Solubility as a percentage
76 %
In g per litre
38 g/L
From nitrogen content
Enter a nitrogen percentage to use the Kjeldahl route
This reports an operational solubility for the conditions you used. True protein solubility depends strongly on pH, ionic strength, temperature and buffer, and is lowest at the isoelectric point.
What this calculator does
Reports how much protein dissolved, as a concentration and as a percentage of what was added.
What your result means
An operational solubility for the exact conditions you used.
Formula and working
Concentration = mass dissolved / volume; solubility % = mass dissolved / mass added × 100
- 1
Collect the inputs
Protein added = 500 mg; Protein recovered undissolved = 120 mg; Solution volume = 10 mL; Measured nitrogen (0 to skip) = 0 %; Nitrogen-to-protein factor = 6.25
- 2
Apply the formula
Concentration = mass dissolved / volume; solubility % = mass dissolved / mass added × 100
- 3
Result
Dissolved protein concentration = 38 mg/mL
Frequently asked questions
Symbols and assumptions
- C
- Concentration
- m
- Mass dissolved
- V
- Volume
- factor
- Nitrogen-to-protein factor
- • Undissolved protein was fully recovered and weighed.
- • Volume is the final solution volume.
Sources and review
Reviewed 2026-09-22 · Next review 2027-09-22
- • True solubility depends strongly on pH, ionic strength, temperature and buffer, and is lowest at the isoelectric point.
Important limitation
This is an educational estimate based only on the values and formula shown. Verify current rates, rules, units, and professional standards before relying on the result. It is not personalized financial or investment advice.
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