Chemistry
Resuspension Calculator
Work out how much buffer to add to a dried pellet or oligo to hit the concentration you want.
Your inputs
Change any value to update the result instantly.
Buffer to add
250 µL
Resulting stock concentration
100 µM
Stock needed for the working dilution
5 µL
Buffer for the working dilution
45 µL
Total amount available
25 nmol
1 nmol in 1 µL is 1 mM, so amounts in nmol and volumes in µL give concentrations in mM; the figures here are shown in µM. Spin the tube down before opening so no dried material is lost.
What this calculator does
Works out the buffer volume needed to resuspend a dried pellet or oligo at a target concentration.
What your result means
The volume to add, plus the stock and diluent needed for a working dilution.
Formula and working
V = n / C
- 1
Collect the inputs
Amount of dried material = 25 nmol; Desired concentration = 100 µM; Working concentration wanted = 10 µM; Working volume needed = 50 µL
- 2
Apply the formula
V = n / C
- 3
Result
Buffer to add = 250 µL
Frequently asked questions
Symbols and assumptions
- V
- Volume
- n
- Amount of substance
- C
- Concentration
- • Amount in nmol and volume in µL, so concentrations follow directly.
- • All the dried material dissolves.
Sources and review
Reviewed 2026-09-22 · Next review 2027-09-22
- • Spin the tube before opening or some dried material is lost, which lowers the real concentration.
Important limitation
This is an educational estimate based only on the values and formula shown. Verify current rates, rules, units, and professional standards before relying on the result. It is not personalized financial or investment advice.
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