Biology
qPCR Efficiency Calculator
Turn the slope of a qPCR standard curve into amplification efficiency and check it against the accepted 90–110% range.
Your inputs
Change any value to update the result instantly.
Amplification efficiency
100.1 %
Amplification factor per cycle
2.001 ×
Cycles to 10× increase
3.32
MIQE-style check (90–110%)
Within the accepted range
A slope of −3.32 means perfect doubling (100% efficiency). Slopes outside roughly −3.1 to −3.6 suggest inhibition, poor primer design or pipetting error in the dilution series.
What this calculator does
Converts a qPCR standard-curve slope into amplification efficiency and checks the accepted 90–110% range.
What your result means
100% efficiency means perfect doubling each cycle (slope −3.32); values outside 90–110% indicate assay problems.
Formula and working
efficiency = (10^(−1 ÷ slope) − 1) × 100%
- 1
Collect the inputs
Standard curve slope = -3.32
- 2
Apply the formula
efficiency = (10^(−1 ÷ slope) − 1) × 100%
- 3
Result
Amplification efficiency = 100.1 %
Frequently asked questions
Symbols and assumptions
- slope
- Slope of Cq versus log₁₀(template) regression
- E
- Efficiency = (10^(−1/slope) − 1) × 100%
- • The slope comes from a serial-dilution standard curve with good R².
- • Efficiency is constant across the dilution range.
Sources and review
Reviewed 2026-09-22 · Next review 2027-09-22
- • A poor R² invalidates the slope; inhibition or primer-dimers distort real amplifications.
Important limitation
This is an educational estimate based only on the values and formula shown. Verify current rates, rules, units, and professional standards before relying on the result. It is not personalized financial or investment advice.
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